Composite

Part:BBa_K4616011

Designed by: Haoyu Zhang   Group: iGEM23_BIT-China   (2023-10-10)


Fluorescent protein detection circuit in E.coli

Usage

This is one of the E.coli fluorescent protein detection gene circuits in the BIT-China 2023 IGEM project "Light's control iT".

igem23-part-rrnbp1-rbs-el222-egfp.png

Figure 1.E.coli transformation experimental verification result. M-marker.2-PBlindv1_ rbs34_eGFP(composite part K4616011,794 bp).

Biology

When el222 gene is expressed, the photosensitive transcription factor EL222 is generated. Under 450nm illumination, the photosensitive transcription factor EL222 is dimerized, binds to the PBLind promoter, and activates the transcription and expression of the downstream green fluorescent gene (egfp).

igem23-part-ecoli-egfp1.png

Figure 2.Light control system in E.coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 789
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 715


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Categories
Parameters
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